polyclonal anti e2f3 Search Results


e2f3  (Bioss)
92
Bioss e2f3
A , B HUVECs were pre-treated with Mas (3 μM) for 2 h and then, LPS was applied to induce inflammatory injury together with Mas for another 48 h. Model group was treated with LPS only for 48 h. Representative western blot analysis to determine the proteins expression of OPA1 in HUVEC cells. C Representative immunofluorescence images for detecting Mfn1/2 (red), DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. D Representative immunofluorescence images for colocalization of DRP-1 (green) and Bak (red) expressions of aorta, DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. E Representative immunofluorescence images for colocalization of TOMM20 (green) and cytochrome c (red) expressions of aorta, DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. F – I HUVECs were pre-treated with Mas (3 μM) for 2 h and then, LPS was applied to induce inflammatory injury together with Mas for another 48 h. Model group was treated with LPS only for 48 h. Representative western blot analysis to determine the proteins expression of Bax, cytochrome c and cleaved-caspase 3 in HUVEC cells. J Representative immumohistochemical images for <t>E2F3</t> expression in aorta (n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. K Representative immumohistochemical images for E2F3 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. L Representative immumohistochemical images for p53 expression in aorta ( n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. M Representative immumohistochemical images for p53 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. N The schematic diagram describes the mitophagy which seems to work as the driving force of DRP-1/Bak/BNIP3-dependent endothelial cells apoptosis. One-way ANOVA was followed by post hoc Tukey’s test. ** P < 0.01 , *** P < 0.001 , vs ctrl group, # P < 0.05 , ### P < 0.001 , vs model group.
E2f3, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e2f3/product/Bioss
Average 92 stars, based on 1 article reviews
e2f3 - by Bioz Stars, 2026-04
92/100 stars
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anti-e2f3 polyclonal antibody 2  (TargetMol)
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anti e2f3 aa 153 409 polyclonal antibody  (Creative BioMart)
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Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5 TTTC CG CGC 3 found in the promoter region of a number of genes whose products are involved in cell
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rabbit anti human e2f3 polyclonal antibody  (Cusabio)
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Rabbit anti Human E2F3 Polyclonal Antibody
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anti-e2f3 polyclonal antibody  (TargetMol)
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anti e2f3 aa 444 457 polyclonal antibody  (Creative BioMart)
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DPABH 27788 detects an 60kDa and 48kDa band in Mouse fetal Lung lysates The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and this band corresponds with results from different antibodies
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rabbit anti acetyl e2f3 polyclonal antibody  (Cusabio)
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Rabbit anti Human Acetyl E2F3 Polyclonal Antibody
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rabbit anti e2f3 polyclonal affinity purified  (Innovative Research Inc)
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Rabbit Anti E2F3 Polyclonal Affinity Purified (PBS with 0.02% sodium azide, 50% glycerol, pH7.3) (Western Blot) from Innovative Research is a polyclonal antibody in a liquid format, buffered in PBS with 0.02% sodium azide, 50%
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anti-e2f3 polyclonal antibody 3  (TargetMol)
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Image Search Results


A , B HUVECs were pre-treated with Mas (3 μM) for 2 h and then, LPS was applied to induce inflammatory injury together with Mas for another 48 h. Model group was treated with LPS only for 48 h. Representative western blot analysis to determine the proteins expression of OPA1 in HUVEC cells. C Representative immunofluorescence images for detecting Mfn1/2 (red), DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. D Representative immunofluorescence images for colocalization of DRP-1 (green) and Bak (red) expressions of aorta, DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. E Representative immunofluorescence images for colocalization of TOMM20 (green) and cytochrome c (red) expressions of aorta, DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. F – I HUVECs were pre-treated with Mas (3 μM) for 2 h and then, LPS was applied to induce inflammatory injury together with Mas for another 48 h. Model group was treated with LPS only for 48 h. Representative western blot analysis to determine the proteins expression of Bax, cytochrome c and cleaved-caspase 3 in HUVEC cells. J Representative immumohistochemical images for E2F3 expression in aorta (n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. K Representative immumohistochemical images for E2F3 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. L Representative immumohistochemical images for p53 expression in aorta ( n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. M Representative immumohistochemical images for p53 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. N The schematic diagram describes the mitophagy which seems to work as the driving force of DRP-1/Bak/BNIP3-dependent endothelial cells apoptosis. One-way ANOVA was followed by post hoc Tukey’s test. ** P < 0.01 , *** P < 0.001 , vs ctrl group, # P < 0.05 , ### P < 0.001 , vs model group.

Journal: Cell Death & Disease

Article Title: Inhibition of PDGFRβ alleviates endothelial cell apoptotic injury caused by DRP-1 overexpression and mitochondria fusion failure after mitophagy

doi: 10.1038/s41419-023-06272-3

Figure Lengend Snippet: A , B HUVECs were pre-treated with Mas (3 μM) for 2 h and then, LPS was applied to induce inflammatory injury together with Mas for another 48 h. Model group was treated with LPS only for 48 h. Representative western blot analysis to determine the proteins expression of OPA1 in HUVEC cells. C Representative immunofluorescence images for detecting Mfn1/2 (red), DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. D Representative immunofluorescence images for colocalization of DRP-1 (green) and Bak (red) expressions of aorta, DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. E Representative immunofluorescence images for colocalization of TOMM20 (green) and cytochrome c (red) expressions of aorta, DAPI (blue) ( n = 4 per group). Scale bar: 50 μm. F – I HUVECs were pre-treated with Mas (3 μM) for 2 h and then, LPS was applied to induce inflammatory injury together with Mas for another 48 h. Model group was treated with LPS only for 48 h. Representative western blot analysis to determine the proteins expression of Bax, cytochrome c and cleaved-caspase 3 in HUVEC cells. J Representative immumohistochemical images for E2F3 expression in aorta (n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. K Representative immumohistochemical images for E2F3 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. L Representative immumohistochemical images for p53 expression in aorta ( n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. M Representative immumohistochemical images for p53 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. N The schematic diagram describes the mitophagy which seems to work as the driving force of DRP-1/Bak/BNIP3-dependent endothelial cells apoptosis. One-way ANOVA was followed by post hoc Tukey’s test. ** P < 0.01 , *** P < 0.001 , vs ctrl group, # P < 0.05 , ### P < 0.001 , vs model group.

Article Snippet: Primary antibodies used were PDGFRβ (Abcam, ab203491), α-SMA (BOSTER, BM0002), OPN (PTG, 22952-1-AP), MOMA (Abcam, ab33451), F4/80 (CST,70076), eNOS (Abcam, ab76198), CD31 (Abcam, ab182981), iNOS (Bioss, BS-0162R), VEGF (Abclonal, A12303), RhoA (Affinity, df5046), ROCK1 (PTG, 21850-1-AP), NOX4 (Abclonal, A22149), DRP-1 (PTG,12957-1-ap), HIF-1α (BOSTER, BM4083), BNIP3 (Abcam, ab109362), LC3B (PTG, 18725-1-AP), Parkin (Bioss, BS-23687), p62 (BOSTER, BA2849), LAMP1 (PTG, 67300-1-IG), TOMM20 (PTG, 11802-1-AP), Mfn1/2 (Abclonal, A12771), Bak (CST, 12105), cytochrome c (CST, 4280 T), E2F3 (Bioss, bs-21438r), p53 (PTG, 21891-1-AP).

Techniques: Western Blot, Expressing, Immunofluorescence, Blocking Assay